Construction and transplantation of tissue-engineered skin with mouse embryonic fibroblasts in SD mice / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the application and mechanism of tissue-engineered skin with mouse embryonic fibroblasts (MEFs) for the full-thickness skin defects on mice.</p><p><b>METHODS</b>The MEFs and fibroblasts were cultured and seeded in scaffold made of rat tail collage. ELISA method was used for detection of secretory function. The full-thickness skin defects were created on mice and covered by MEFs-scaffold complex (experimental group), or FBs-scaffold complex (control group 1), or scaffold only (control group 2). The process of wound healing was evaluated by observation of the re-epithelization rate. Microvessel density (MVD) and vimentin within the wound sites were also detected with immunohistochemistry staining technique to describe the characteristics of wound healing. Hoechst 33342 staining was performed to trace MEFs'fate.</p><p><b>RESULTS</b>MEFs scaffold group had higher level secretion of IL-6 and lower of TGF-beta1 than FBs scaffold group (P<0.05). Compared with wounds in control groups, the wounds in MEFs group healed markedly fast (P<0.05) and the MVD was significantly higher (P <0.05). The fibroblasts in the wounds of MEFs group were arranged regularly and the MEFs decreased during the healing process.</p><p><b>CONCLUSIONS</b>The MEFs-scaffold complex can promote wound healing with less scar formation. MEFs may have an inducing effect on the wound healing.</p>

Full face dermabrasion for acne scars / 中华整形外科杂志

<p><b>OBJECTIVE</b>Dermabrasion has been of great value in plastic surgery. Dermabrasion was developed for a specific indication; however, within a very short time, the concept of dermabrasion found wide applicability. This study was to investigate the application of dermabrasion in the treatment of acne scars.</p><p><b>METHODS</b>From Feb. 1996 to May 2004, a total of 110 patients with acne scar were treated with dermabrasion.</p><p><b>RESULTS</b>Postoperatively, the curative results were achieved in 45 cases; good results in 40 cases and effective results in 25 cases. The study revealed that the patients at 18-46 years of age have good results.</p><p><b>CONCLUSIONS</b>Dermabrasion is a good and safe technique to treat the scar of acne.</p>

Tissue expansion under the cicatrix / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate a more simple and effective method to repair cicatrix by tissue expansion.</p><p><b>METHODS</b>The dilator with the capacity of 80 - 500 ml was implanted into the subcutaneous pocket under the cicatrix. After dilating for one to two months, the dilator was taken out and the wound surface of the cicatrix was removed. The expanded skin flap was advanced or rotated to cover the defects. The procedure was used on 203 cases.</p><p><b>RESULTS</b>The dilatation was achieved successfully in all the cases, followed by cicatrix removing and repair. The incision scar was not noticeable.</p><p><b>CONCLUSIONS</b>Tissue expansion under the cicatrix has the advantages of safety, less trauma and less extra incisions. It is a reasonable choice to obtain more flexible surgical designs and more economical skin flap applications. It is suitable for most of the treatment for cicatrix.</p>

Minimizing the antigenicity of tracheal allografts by using a technique of cryopreservation / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the proper time of cryo-preserving tracheal allograft so as to minimize its antigenicity.</p><p><b>METHODS</b>On a dog model, this study was carried out by allografting a tracheal into a muscular flap formed with sternocephalic muscle and sternohyoid--sternothyroid muscle. The tracheal was treated with cryopreservation in defferent intervals. The viability of the graft was evaluated by the examination of fiberoptic bronchoscopy, histopathology and microangiography. The blood flow of the tracheal mucous was measured with a blood flowmeter and the survival area was decided in the calculation of the percentage.</p><p><b>RESULTS</b>There are no significant differences in the mucous membrane appearance and the mucosal blood flow one week after the surgery among the non-cryopreservation group and the groups treated with cryopreservation in 1 day, 2 weeks, 4 weeks, 6 weeks and 8 weeks. The graft was found to start necrosis 2 weeks after the transplantation with the infiltration of mononuclear cells examined under light microscope in almost all of the groups, especially in the non-cryopreservation group and the groups treated with cryopreservation in 1 day, 2 weeks. However, there was no significant difference among the autograft group and the allograft groups cryopreservated in 6 weeks and 8 weeks, and the infiltration of the mononuclear cells was not found in these groups either.</p><p><b>CONCLUSION</b>The antigenicity of the tracheal allografts could be significantly decreased by the treatment of cryopreservation over 6 weeks.</p>

Cleft lip nasal deformity corrected by an alar flap and the alar cartilage sling method / 中国医学科学院学报

<p><b>OBJECTIVE</b>To search an effective method to correct the secondary nasal deformity.</p><p><b>METHODS</b>The "spilth" tissue asymmetry to the another side on the cleft side alar is formed as a flap, which is used to drive up or reconstruct the nostril base (sill), readjust nostril size and shape. The cleft side alar cartilage lateral foot is disassociated, replaced and fixed into the normal place.</p><p><b>RESULTS</b>Nineteen patients were received this operation, their nasal alar, nostril, sill, on the two sides are symmetry, and the result is good.</p><p><b>CONCLUSIONS</b>The cleft side alar flap and alar cartilage sling procedure is effective to correct secondary cleft lip nasal deformity.</p>

The investigation of tracheas transplantation by wrapping in a muscle flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the possibility of tracheas transplantation by wrapping it in a muscle flap.</p><p><b>METHODS</b>With a dog model, a number of tracheas were separately wrapped in the unilateral sternocephalic muscle flap and the bilateral sternohyoid-sternothyroid muscle flap, and placed in the original site. The tracheas autografting was used as a control. The viability was evaluated by the examination of fiberoptic bronchoscopy, histopathology and microangiography, the measurement of tracheal mucosal blood flow and the calculation of survival rate and percentage of patency.</p><p><b>RESULTS</b>The submucosal blood flow of the transplanted tracheas was detected in the unilateral sternocephalic muscle flap group and the bilateral sternohyoid-sternothyroid muscle flap group 1 week after the surgery and gradually reached the level close to the normal in 4 weeks, while the vascular ingrowth was also shown from the wrapped muscle flap into the transplanted tracheas by using a microangiography technique. The histopathological examination demonstrated that the structure of the transplanted tracheas was quite same as the original one and its inner surface was also covered with pseudostratified columnar ciliary epithelia. However, in the control group, the mucous membranes turned black one week after the transplantation and all dogs died from the graft necrosis.</p><p><b>CONCLUSION</b>The tracheas wrapped in a muscular flap could survive well for a long time.</p>